catalog number : byk-2188r
quantity size : 0.1ml (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
background: glyceraldehyde 3 phosphate dehydrogenase (gapdh) is well known as one of the key enzymes involved in glycolysis. as well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian gapdh is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear rna export, dna replication, and dna repair. during the last decade a lot of data appeared concerning the role of gapdh in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as alzheimer's and huntington's disease. gapdh is expressed in all cells. it is constitutively expressed in almost all tissues at high levels. there are however some physiological factors such as hypoxia and diabetes that increase gapdh expression in certain cell types. gapdh molecule is composed of four 38kda subunits.
specificity :
anti-gapdh is a rabbit polyclonal antibody unconjugated specific for gapdh of human,mouse,rat,use for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 37kda
application :
western blotting=1:100-500 immunohistocry 1:100-500 elisa= 1:500-1000 ihc-p=1:100-500ihc-f=1:100-500optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.