作者：xiaogangpana,b,jingjiaoguanb,c,jung-wooyood,arthurj.epsteind,e,l.jamesleeb,c,robertj.leea,b,f
adivisionofpharmaceutics,collegeofpharmacy,theohiostateuniversity,columbus,oh43210,unitedstates
bnsfnanoscalescienceandengineeringcenter(nsec),centerforaffordablenanoengineeringofpolymericbiomedicaldevices(canpbd),theohiostateuniversity,columbus,oh43210,unitedstates
cdepartmentofchemicalandbiomolecularengineering,theohiostateuniversity,columbus,oh43210,unitedstates
ddepartmentofphysics,theohiostateuniversity,columbus,oh43210,unitedstates
ecenterformaterialsresearch,theohiostateuniversity,columbus,oh43210,unitedstates
fncicomprehensivecancercenter(ccc),theohiostateuniversity,columbus,oh43210,unitedstates
摘要：cationiclipid-coatedmagneticnanoparticles(mps)associatedwithtransferrinwereevaluatedasgenetransfervectorsinthepresenceofastaticmagneticfield.mpswerepreparedbychemicalprecipitationandweresurface-coatedwithcationiclipids,composedofddab/soypc(60:40mole/mole).thesecationicmpswerethencombinedwithpolyethylenimine(pei)condensedplasmiddna,followedbytransferrin.theresultingmagneticelectrostaticcomplexesretainedrelativelycompactparticlesizeandshowedcompletednacondensation.theirtransfectionactivityinthepresenceofastaticmagneticfieldwasevaluatedbyluciferaseandgreenfluorescentprotein(gfp)reportergenes.themagneticcomplexesexhibitedupto300-foldhighertransfectionactivitycomparedtocommonlyusedcationicliposomesorcationicpolymercomplexes,basedonluciferaseassay.theenhancementintransfectionactivitywasmaximizedwhenthecellswereexposedtothevectorsforarelativelyshortperiodoftime(15min),orweretreatedinmediacontaining10%serum.incorporationoftransferrinfurtherimprovedtransfectionefficiencyofthecationicmps.however,whencellswereincubatedfor4hinserum-freemedia,magneticandnon-magneticvectorsshowedsimilartransfectionefficiencies.inconclusion,transferrin-associatedcationicmpsareexcellentgenetransfervectorsthatcanmediateveryrapidandefficientgenetransferinvitrointhepresenceofamagneticfield.
关键词：genedelivery;magneticnanoparticles;transferrin;cationiclipid;magnetofection