[100 µl]
catalog number: 21019
gene symbol: cdc42
description: anti-cdc42 chicken polyclonal antibody
background: small gtpases are a super-family of cellular signaling regulators. cdc42 belongs to the rho sub-family of gtpases that regulate cell motility, cell pision, and gene transcription. gtp binding increases the activity of cdc42, and the hydrolysis of gtp to gdp renders it inactive. gtp hydrolysis is aided by gtpase activating proteins (gaps), while exchange of gdp for gtp is facilitated by guanine nucleotide exchange factors (gefs).
immunogen: recombinant full length cdc42 protein
applications: elisa, wb, ihc
recommended dilutions:
elisa: 1:100-1:5000
wb: 1:100-1:1000
ihc: 1:100-1:1000
concentration: 1 mg/ml
host: chicken
format: liquid
clonality: polyclonal
isotype: igy
purity: purified from egg yolk
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: cdc42 antibody recognizes cdc42 from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
western blot:
western blot analysis of cdc42 proteins. hepg whole-cell lysate was used for western blot with the chicken polyclonal anti-cdc42 antibody (cat # 21019).
publications:
1.
plos pathog. 2013 jan;9(1):e1003142
2.
cytokine. 2012 jan;57(1):158-68
3.
european journal of cell biology volume 91, issues 11–12, november–december 2012, pages 978–987
4.
j cell biol. 2012 aug 20;198(4):677-93
5.
am j physiol lung cell mol physiol. 2011 jan;300(1):l32-42
6.
mol cell biol. 2011 nov;31(22):4430-41
7.
plos one. 2013 jul 19;8(7):e70123
8.
plos one. 2013 sep 3;8(9):e73063
9.
j biol chem. 2014 jun 27;289(26):18347-59
10.
united states patent application 20140194451
11.
molecular pharmacology january 2014 vol. 85 no. 1 50-61
武汉纽斯特生物技术有限公司
18972339958
中国 武汉