比利时伊氏锥虫病（苏拉病）乳胶凝集试纸卡
250份/盒伊氏锥虫病(又称“苏拉病”)是由伊氏锥虫引起的一种血液原虫病，多发于热带和亚热带地区。临床特征为进行性消瘦、贫血、黄疸、高热、心肌能衰竭，常伴发体表水肿和神经症状等。0ie将其列为b类疫病。试剂盒含有完成伊氏锥虫卡片凝集试验检测全过程所需全部试剂：抗原、缓冲液、阳性血清和阴性血清；可对马属动物、猪、牛、羊等多种动物的血清进行检测
cardagglutination test for animal trypanosomiasis
causedby t.evansi
thisfield and laboratory test (catt/t.evansi) isused for the serodiagnosis of animal african trypanosomiasis (t.evansi). it is a direct card agglutination test for detectionof trypanosome specific antibodies in blood, serum or plasmaof t.evansi in different animalspecies.
it iscurrently applied for serodiagnosis of t.evansi invarious host species (i.e. camels. buffaloes, equidae,dogs,…)
reagent | freeze driedsuspension of purified, fixed and stained trypanosomes of a clonedpredominant variable antigen type (vat rotat 1.2)of t.evansi.
test sample | whole blood (for screening), plasma (onheparine or edta) or serum.
shelf life - expiry date | when stored under theprescribed storage conditions, all the reagents will retain theiractivity until the expiry date mentioned on the “reagent” boxes andon the packing list (1 year).
storage
·        freeze dried reagents (antigen, controls) : in therefrigerator(+2°c to +8°c) or in the freezer (-20°c)
·        catt buffer: in the refrigerator (+2°c to +8°c) – do notfreeze!
·        during transport, storage and handling: avoid exposure to heat anddirect sunlight.
·        it is recommended to dispatch the reagents from a central storagecentre to the field under refrigerated conditions (coldchain).
packaging
testkit containing all reagents and accessory materials +protocol.
·         catt/tevt250: 250 screening tests
what else do youneed?
·        card test rotator
references
·         c.gutierrez, m.c. juste, j.a. corbera, e. magnus, d. verloo and j.a.montoya (2000): camel trypanosomosis in the canaryislands: assessment of seroprevalence and infection rates using thecard agglutination test (catt/t.evansi) and parasitedetection tests.  vet. parasitol., 90, 155-159.
·         d.verloo, e. magnus and p. büscher (2001): generalexpression of rotat 1.2 variable antigen type in trypanosoma evansiisolates from different origin. vet. parasitol., 97,183-189.
·         w.g.holland, n.g.thanh, l.n. my, e. magnus, d. verloo, p. büscher, b.goddeeris and j. vercruysse (2002): evaluation of wholefresh blood and dried blood on filter paper disks in serologicaltests for trypanosoma evansi in experimentallyinfected water buffaloes.  acta tropica, 81,159-165.

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