conditions
column: ace 3 c18
dimensions: 100 x 4.6 mm
part number: ace-111-1046
mobile phase: a: 3 mm tetrabutylammonium bromide and 5 mm kh2po4 in h2o
b: 5 mm tetrabutylammonium bromide in meoh
gradient:
time(mins)
%b
0
45
20
70
30
45
40
45
flow rate: 0.8 ml/min
injection: 10 μl
temperature: ambient
detection: uv-vis, 420 nm, 520 nm and 600 nm
analytes
1. amaranth
2. sunset yellow
3. allura red
4. red 2g
5. ponceau 4r
6. carmoisine
7. erythrosine