使用zeta life，advanceddnarna转染试剂
高效率转染hct-116, sw480, ht-29人结直肠癌细胞
2020-4-28发表文章已见刊
发表文章转染条件
a、hct-116, sw480, ht-29人结直肠癌细胞
b、aurka质粒
c、转染细胞融合度50%
d、96孔板每孔使用0.5μg质粒dna
6孔每孔使用10 ug质粒dna
(注意：本实验中用到的细胞密度、转染质粒dna用量不适用于lipo3000/2000)
发表文章部分内容
overexpression of aurkaplasmid for aurka overexpression was obtained fromvectorbuilder. details about plasmid containing the aurka gene canbe found at colon cancer cells (hct-116, sw480, ht-29)were seeded at 50% confluency for 24 h in six-well and 96-well plates. plasmid (10 ug per-well for six-well and 0.5μg per-well for96 plates) and theadvanceddnarnatransfection（zeta life,usa) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. dox (1μg/ml) was addedto induce the expression of aurka. after addition of dox and pal for36 h, cell viability was measured by mtt and apoptosis was detected by。
pal exerts anti-colon cancer effects by targeting aurka. (a) pal promotes apoptosis in colon cancer cells (hct-116, sw480) in a dose-dependent manner.(b) pal promotes g2/m phase arrest in colon cancer cells (hct-116, sw480) in a dose-dependent manner. (c) the expression of aurka after dox treatment for24 h. (d) cell density after pal treatment for 24 h in the cells (hct-116, sw480) transfected with aurka overexpression plasmids. dox is an inducer of aurka
expression in plasmids. (e) effect of pal detected by mtt assay on the proliferation ability of colon cancer cells transfected with aurka overexpression plasmids. (f)effects of pal detected by.
zeta life，advanced dna rna转染试剂信息如下：
产品名称
货号
规格
advanced dna rna 转染试剂
ad600025
0.25ml
advanced dna rna 转染试剂
ad600050
0.5
advanced dna rna 转染试剂
ad600075
0.75
advanced dna rna 转染试剂
ad600150
1.5ml
advanced dna rna 转染试剂
ad600500
5ml
advanced dna rna 转染试剂
ad601000
10ml