文献名：cytosolicdeliveryofmembrane-impermeablemoleculesindendriticcellsusingph-responsivecore−shellnanoparticles
作者：yuhuahu,tamaralitwin,arpunr.nagaraja,brandonkwong,joshuakatz,nickiwatson,darrellj.irvine
departmentofchemicalengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofchemistry,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofmaterialsscienceandengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,departmentofbiologicalengineering,massachusettsinstituteoftechnology,77massachusettsavenue,cambridge,massachusetts02139,andwhiteheadinstitute,9cambridgecenter,cambridge,massachusetts02142
摘要：polycationsthatabsorbprotonsinresponsetotheacidificationofendosomescantheoreticallydisruptthesevesiclesviathe“protonsponge”effect.toexploitthismechanism,wecreatednanoparticleswithasegregatedcore−shellstructureforefficient,noncytotoxicintracellulardrugdelivery.cross-linkedpolymernanoparticlesweresynthesizedwithaph-responsivecoreandhydrophilicchargedshelldesignedtodisruptendosomesandmediatedrug/cellbinding,respectively.bysequesteringtherelativelyhydrophobicph-responsivecorecomponentwithinamorehydrophilicph-insensitiveshell,nontoxicdeliveryofsmallmoleculesandproteinstothecytosolwasachievedindendriticcells,akeycelltypeofinterestinthecontextofvaccinesandimmunotherapy.