conditions
column: ace excel 2 superc18
dimensions: 100 x 3.0 mm
part number: exl -1011-1003u
mobile phase:
a: 20 mm ammonium formate ph 3.0 in h2o
b: 20 mm ammonium formate ph 3.0 in meoh/h2o (9:1 v/v)
gradient:
time(mins)
%b
0.0
50
6.0
70
7.0
70
7.5
50
flow rate: 1.2 ml/min
injection: 2 μl
temperature: 40 °c
detection: uv, 260 nm
analytes
1. doxepin + isomer
2. imipramine
3. desipramine
4. amitriptyline
5. nortriptyline
6. clomipramine