coffee metabolite profiling by lc-ms
conditions
column: ace excel 1.7 c18-amide
dimensions: 100 x 2.1 mm
part number: exl-1712-1002u
mobile phase:
a: 0.01% formic acid in h2o
b: 0.01% formic acid in mecn
time(mins)
%b
0.0
3
2.5
10
8.0
100
8.5
3
10.0
3
flow rate: 0.5 ml/min
detection: exactive accurate mass ms system esi in negative ion mode analytes between m/z 70-800 monitored
sample: metabolites from coffee extracted into cold water by vortexing for 20 mins.
samples filtered prior to injection onto column and modular accela lcsystem