在豚鼠咳嗽模型中反复柠檬酸暴露可引起气道重塑和持续咳嗽
shilei cuia,b, isao itoa,?, hitoshi nakajia, toshiyuki iwataa, hisako matsumotoa, tsuyoshi ogumaa,
tomoko tajiria, tadao nagasakia, yoshihiro kanemitsua,c, hiromi izuharaa, michiaki mishimaa,akio niimia,c
a department of respiratory medicine, graduate school of medicine, kyoto university, japan
b department of respiratory medicine, huadong hospital, fudan university, shanghai, china
c department of medical oncology and immunology, nagoya city university, graduate school of medical science, japan
a b s t r a c t
background:a previous study involving guinea pigs showed that repeated cough could increase peripheral airway smooth muscle area, which can also aggravate cough. the airway pathologic changes produced by prolonged cough are still unknown.
背景：先前一项涉及豚鼠的研究表明，反复咳嗽可增加外周气道平滑肌面积，也可加重咳嗽。长期咳嗽引起的气道病理改变尚不清楚。
objective:to study the airway pathologic changes in prolonged cough models of guinea pigs.
目的：研究豚鼠长期咳嗽模型气道病理变化。
methods:guinea pigs were assigned to three treatment groups: citric acid inhalation (ca) alone, citric acid inhalation with codeine pretreatment (cod), or saline solution inhalation (sa). animals were challenged with citric acid or saline solution three times weekly. the intervention period was 22 or 43 days. animals were challenged with citric acid on the first and last days of exposure. lung specimens were obtained for pathologic analysis 72 h after the last exposure.
方法：将豚鼠分为3个治疗组：单独吸入柠檬酸(ca)、可待因预处理柠檬酸(cod)和盐水吸入(sa)。每周用柠檬酸或盐水对动物进行三次激发。干预期为22、43天。在暴露的天和后一天，用柠檬酸对动物进行挑战。后一次暴露72h后取肺标本进行病理分析。
results: compared with the other two groups, the ca group had increased frequency of cough on both 22 and 43 days of exposure. tracheal basement membrane (bm) thickness was increased after 43 days of exposure, correlating with the frequency of cough. the area of airway smooth muscles (asm index) in small airways increased in the ca group after both 22 and 43 days of exposure, compared with the sa group. compared with the cod group, the asm index in small airways increased in the ca group after 22 days of exposure instead of 43 days of exposure.
结果：与其他两组相比，ca组在暴露22天和43天时咳嗽频率均增加。暴露43天后，气管基膜(bm)厚度增加，与咳嗽频率相关。暴露22和43天后，ca组气道平滑肌面积(asm指数)均较sa组增加。与cod组相比，ca组暴露22天后小气道asm指数升高，而非43天。
conclusions:an increase in peripheral smooth muscle area by repeated cough was confirmed. moreover, this is the first study to show that tracheal bm thickness increased after prolonged exposure (43 days). repeated cough may lead to airway remodeling, which was also associated with an increased frequency of cough.
结论：反复咳嗽可增加外周平滑肌面积。此外，这是*研究表明，气管骨厚度增加后，长期暴露(43天)。反复咳嗽可能导致气道重塑，这也与咳嗽频率增加有关。
keywords:cough、cough reflex sensitivity、airway remodeling、mechanical stress
关键词：咳嗽、咳嗽反射敏感性、气道重塑、机械应激