from:abcam
catalognumber:rs-0091r
quantitysize:100ul(dilutewithph7.40.01mpbsorantibodydiluent)
background:
muc16(ca125)isaserummarkerthatisusedroutinelyingynecologicpracticetomonitorpatientswithovariancancer.itisamullerianductdifferentiationantigenthatisoverexpressedinepithelialovariancancercellsandsecretedintotheblood,althoughitsexpressionisnotentirelyconfinedtoovariancancer.mostbiochemicalstudieshaveconcludedthatmuc16isahighmolecularmassglycoprotein,althoughestimatesofitssizerangefrom200to2000kdawithsmaller“subunits”beingdescribedbysomeinvestigators.
function:
thoughttoprovideaprotective,lubricatingbarrieragainstparticlesandinfectiousagentsatmucosalsurfaces.
subunit:
bindstomsln.bindingtomslnmediatesheterotypiccelladhesion.thismaycontributetothemetastasisofovariancancertotheperitoneumbyinitiatingcellattachmenttothemesothelialepitheliumviabindingtomsln.
specificity:anti-ca125isamousemonoclonalantibody
specificforanti-ca125ofrat,human,mouse,
-useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry
proteingaffinitychromatographypurification,purity:>95%isotype:iggmolwt:200kda
application:
wb=1:100-500elisa=1:500-1000ihc-p=1:100-500ihc-f=1:100-500optimaldilutions/concentrationsshouldbedeterminedbytheenduser..klhconjugatedsyntheticpeptidederivedfromhumanca125
storage:storeat-20℃foroneyear.avoidrepeatedfreeze/thawcycles.thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20℃.whenreconstitutedinsterileph7.40.01mpbsordiluentofantibody,theantibodyisstableforatleastsixweeksat2-4℃
importantnote:thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.