作者：neerav.gopee*†,deanw.roberts*†,peggywebb*†,christyr.cozart*,paulh.siitonen*,alanr.warbritton†,williamw.yu§,vickil.colvin§,nigelj.walker¶andpaulc.howard*†
*nationalcenterfortoxicologicalresearch
†nationaltoxicologyprogramcenterforphototoxicology,u.s.foodanddrugadministration,jefferson,arkansas72079
†toxicologypathologyassociates,jefferson,arkansas72079
§centerforbiologicalandenvironmentalnanotechnologyanddepartmentofchemistry,riceuniversity,houston,texas,77251
¶nationalinstituteofenvironmentalhealthsciences,nationalinstitutesofhealth,andthenationaltoxicologyprogram,researchtrianglepark,northcarolina,27709
摘要：topicalexposuretonanoscalematerialsislikelyfromavarietyofsourcesincludingsunscreensandcosmetics.becausetheinvivodispositionofnanoscalematerialsisnotwellunderstood,wehaveevaluatedthedistributionofquantumdots(qds)followingintradermalinjectionintofemaleskh-1hairlessmiceasamodelsystemfordeterminingtissuelocalizationfollowingintradermalinfiltration.theqd(cdsecore,cdscapped,poly[ethyleneglycol]coated,37nmdiameter,621nmfluorescenceemission)wereinjectedintradermally(id)ontherightdorsalflank.withinminutesfollowingintradermalinjection,thehighlyuvfluorescentqdcouldbeobservedmovingfromtheinjectionsitesapparentlythroughthelymphaticductsystemtoregionallymphnodes.residualfluorescentqdremainedatthesiteofinjectionuntilnecropsyat24h.quantificationofcadmiumandseleniumlevelsafter0,4,8,12,or24hinmultipletissues,usinginductivelycoupledplasmamassspectrometry(icp-ms),showedatime-dependentlossofcadmiumfromtheinjectionsite,andaccumulationintheliver,regionaldraininglymphnodes,kidney,spleen,andhepaticlymphnode.fluorescencemicroscopycorroboratedtheicp-msresultsregardingthetissuedistributionofqd.theresultsindicatedthat(1)idinjectednanoscaleqdremainedasadepositinskinandpenetratedthesurroundingviablesubcutis,(2)qdweredistributedtodraininglymphnodesthroughthesclymphaticsandtotheliverandotherorgans,and(3)sentinelorgansareeffectivelocationsformonitoringtransdermalpenetrationofnanoscalematerialsintoanimals.
关键词：nanoparticles,nanoscalematerials,quantumdots,sentinelorgans,biodistribution