作者：shefahqazi††,larso.liepold††,mdjoynalabedin††,benjohnson††,peterprevelige ,josepha.frank #,andtrevordouglas*††
†chemistryandbiochemistrydepartmentand†centerforbio-inspirednanomaterials,montanastateuniversity,bozeman,montana,59717,unitedstates
departmentofmicrobiology,universityofalabamaatbirmingham,birmingham,alabama35294,unitedstates
franklaboratory,radiologyandimagingsciences,clinicalcenter,and#nationalinstituteofbiomedicalimagingandbioengineering,nationalinstitutesofhealth,bethesda,maryland20892,unitedstates
摘要：attachmentofmultiplechelatedgd3+ionstotheinteriorofbacteriophagep22viralcapsidsaffordsnanoscalemricontrastagentswithextremelyhighrelaxivityvalues.highlyfenestrated“wiffleball”morphologyisuniquetop22andassureswaterexchangebetweentheenvironmentandinteriorcavityofthecapsid.thecavityofp22“wiffleball”wasfunctionalizedwithabranchedoligomercomprisingmultipledtpa–gdcomplexesresultinginanimpressivepayloadof1,900gd3+ionsinsideeach64nmcapsid.highrelaxivitiesofr1,ionic=21.7mm–1s–1andr1,particle=41,300mm–1s–1at298k,0.65t(28mhz)arereported,withr1/r2ratioof0.80andoptimizedrotationalcorrelationtimeforthissystem.specificdesignmodificationsaresuggestedforfutureimprovementsofviralcapsid-basedmricontrastagentsdirectedtowardclinicaltranslation.
关键词：viralcapsid,vlp,bacteriophagep22,magneticresonanceimaging,mri,imagingagent,contrastagent,t1,relaxivity,gadolinium,dtpa-gd,azide-alkyneclickchemistry,nanoparticle,nanocomposite,proteincages,payload