nmt是基因功能的活体检测技术，已被103位诺贝尔奖得主所在单位，及北大、清华、中科院使用。
nmt历*的今天
2014年01月14日，中科院水生所王强、陈辉用nmt在plant and cell physiology上发表了标题为ca2+ signal transduction related to neutral lipid synthesis in an oil-producing green alga chlorella sp. c2的研究成果。'
期刊：plant and cell physiology
主题：ca2+流指示的微藻氮胁迫信号转导研究
标题：ca2+ signal transduction related to neutral lipid synthesis in an oil-producing green alga chlorella sp. c2
影响因子：4.134
检测指标：ca2+流速
作者：中科院水生所王强、陈辉
英文摘要
changes in the cytosolic ca2+ levels and the role of ca2+ signal transduction in neutral lipid synthesis in chlorella sp. c2 under nitrogen starvation conditions were investigated. the results detected by using the scanning ion-selective electrode technique demonstrate that nitrogen starvation induced significant ca2+ influx across the plasma membrane into cells.
ca2+ fluorescence imaging and flow cytometry were used to estimate the effect of this ca2+ influx on the generation of the ca2+ signal, and the results showed that the cytosolic ca2+ concentration increased transiently and then remained at a stable, high level when the cells were exposed to nitrogen starvation. however, the increase could be inhibited by pre-treatment with the ca2+ channel blockers ruthenium red, verapamil and gdcl3, indicating that both the influx of ca2+ from the extracellular space via ca2+ channels that are localized in the plasma membrane and the release of ca2+ from intracellular calcium storage via the internal calcium store were required for the generation and transduction of the ca2+ signal.
during nitrogen starvation, neutral lipid synthesis in chlorella sp. c2 in response to stress conditions was also inhibited to differing degrees by pre-treatment with the three ca2+ channel blockers, demonstrating the regulation of ca2+ via these ca2+ channels in neutral lipid synthesis.
the results suggested that by transduction of extracellular stress signals into the cell and the regulation of the ca2+ signal in neutral lipid synthesis, ca2+ signal transduction played important roles in the response mechanism of chlorella sp. c2 to nitrogen starvation.
中文摘要（谷歌机翻）
小球藻中胞质ca2+水平的变化以及ca2+信号转导在中性脂质合成中的作用。研究了氮饥饿条件下的c2。通过使用扫描离子选择电极技术检测到的结果表明，氮饥饿导致大量ca2+穿过质膜流入细胞。
ca2+荧光成像和流式细胞仪用于评估这种ca2+内流对ca2+信号生成的影响，结果表明，当细胞暴露于细胞中时，胞浆中ca2+的浓度会瞬时增加，然后保持稳定的高水平。氮饥饿。但是，通过用ca2+通道阻滞剂钌红，维拉帕米和gdcl3进行预处理可以抑制这种增加，这表明ca2+经由位于质膜上的ca2+通道从细胞外空间大量涌入以及ca2+的释放。ca2+信号的产生和转导需要通过内部钙储存器进行细胞内钙储存。
在氮饥饿期间，小球藻中的中性脂质合成。通过使用三种ca2+通道阻滞剂的预处理，c2对应激条件的响应也受到了不同程度的抑制，这表明在中性脂质合成中通过这些ca2+通道对ca2+的调节。
结果表明，通过将细胞外应激信号转导到细胞中以及中性脂质合成中ca2+信号的调节，ca2+信号转导在小球藻的反应机制中发挥了重要作用。c2导致氮饥饿。
fig. 1 the total ca2+ flux rates over 5 min in chlorella sp. c2 under n starvation. (a) microphotographic examples of ca2+ ion flux/voltage-clamp measurements. (b) total flux rates of ca2+ were detected at 0, 0.5, 2 and 8 d after n starvation. the columns represent the means of three replicated studies in each sample, with the sd of the means (t-test, p < 0.05). the significance of the differences between the control (0 d) and other test values was tested using a one-way analysis of variance. *p < 0.05 vs. control.