conditions
column: ace 5 c18
dimensions: 250 x 4.6 mm
part number: ace-121-2546
mobile phase: 50 mm kh2po4 ph 3.0 in h2o/meoh (97:3 v/v)
flow rate: 1 ml/min
temperature: ambient
detection: uv, 205 nm
analytes
1. pyridoxamine
2. thiamine (vitamin b1)
3. l -ascorbic acid (vitamin c)
4. nicotinic acid
5. pyridoxal
6. impurity
7. pyridoxine