conditions
column: ace 5 c18
dimensions: 150 x 3.0 mm
part number: ace-121-1503
mobile phase: 10 mm oxalic acid ph 2.9/mecn(80:20 v/v)
flow rate: 0.5 ml/min
injection: 20 μl
temperature: ambient
detection: uv-vis, 350 nm
analytes
1. oxytetracycline
2. tetracycline
3. demeclocycline
4. chlortetracycline
5. doxycycline