liquorice extracts fingerprint
conditions
column: ace 3 c18-pfp
dimensions: 150 x 2.1 mm
part number: ace-1110-1502
mobile phase:
a: ammonium acetate in h2o ph 4
b: meoh
time(mins)
%b
0
10
1
10
11
15
55
90
60
100
flow rate: 0.4 ml/min
injection: 2 μl
temperature: 40 °c
detection: uv, 260, 280, 330 and 350 nm
sample: plant material ground to a fine powder in pestle and mortar. powdered material extracted into methanol by ultrasonification for 30 minutes, followed by centrifugal filtration.
methanolic liquorice leaf extract at different wavelengths
comparison of methanolic extracts at 260 nm