上海研盟生物科技有限公司有标记的一抗出售,相关标记有:alexa fluor 350 标记、alexa fluor 488 标记、alexa fluor 555 标记、alexa fluor 647 标记、ap标记、apc标记、biotin标记、cy3标记、cy5标记、cy5.5标记、cy7标记、fitc标记、gold标记、hrp标记、pe标记、pe-cy3标记、pe-cy5标记、pe-cy5.5标记、pe-cy7标记、rbitc标记 核糖体蛋白l23a抗体,anti-rpl23a抗体产品介绍:ribosomes, the organelles that catalyze protein synthesis, consist of a small 40s subunit and a large 60s subunit. together these subunits are composed of 4 rna species and approximately 80 structurally distinct proteins. this gene encodes a ribosomal protein that is a component of the 60s subunit. the protein belongs to the l23p family of ribosomal proteins. it is located in the cytoplasm. the protein may be one of the target molecules involved in mediating growth inhibition by interferon. in yeast, the corresponding protein binds to a specific site on the 26s rrna. this gene is co-transcribed with the u42a, u42b, u101a, and u101b small nucleolar rna genes, which are located in its third, first, second, and fourth introns, respectively. as is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. 请来电咨询:021-60895578 或者添加qq:1764881598 直接索取说明书 中文名称:核糖体蛋白l23a抗体 英文名称:anti-rpl23a antibody 产品编号:byk-19188r 规格:0.1ml/0.2ml 浓度:1mg/1ml 核糖体蛋白l23a抗体保存条件: store at -20 °c for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°c. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody the antibody is stable for at least two weeks at 2-4 °c. 抗体来源:本公司抗体有兔来源和鼠来源等 克隆类型:兔来源为多抗,鼠来源为单抗 产品类型:一抗 anti-rpl23a抗体别名:60s ribosomal protein l23a; flj27455; l23a; mda20; melanoma differentiation-associated gene 20; ribosomal protein l23a; rl23a_human ; rpl23a. 请来电咨询:021-60895578 或者添加qq:1764881598 直接索取说明书 核糖体蛋白l23a抗体,anti-rpl23a抗体石蜡切片免疫组化染色步骤
1、三步法(以sp试剂盒为例)
- 石蜡切片脱蜡至水
- 蒸馏水冲洗,pbs浸泡5分钟,如果采用抗原修复,可在此步后进行
- 3% h2o2 室温孵育5~10分钟,以消除内源性过氧化物酶的活性,pbs冲洗,2分钟×3次
- 5~10%正常山羊血清封闭,室温孵育10分钟。倾去血清,勿洗,滴加适当比例稀释的一抗或一抗工作液,37℃孵育1~2小时或4℃过夜
- pbs冲洗,2分钟×3次
- 滴加适当比例稀释的生物素标记二抗(1%bsa-pbs稀释),37℃孵育10~30分钟;或滴加第二代生物素标记二抗工作液,37℃或室温孵育10~20分钟
- pbs冲洗,2分钟×3次
- 滴加适当比例稀释的辣根酶标记链霉卵白素(pbs稀释),37℃孵育10~30分钟;或第二代辣根酶标记链霉卵白素工作液,37℃或室温孵育10~20分钟
- pbs冲洗,2分钟×3次
- 显色剂显色(dab或aec)
- 自来水充分冲洗,复染,脱水透明、封片
- 如果必要,可选用avdin封闭内源性生物素
请来电咨询:021-60895578 或者添加qq:1764881598 直接索取说明书 核糖体蛋白l23a抗体,anti-rpl23a抗体2.sabc法
- 脱蜡、水化
- pbs洗2次各5分钟
- 用蒸馏水或pbs配制新鲜的3% h2o2 ,室温封闭5-10分钟,用蒸馏水洗3次
- 抗原修复
- pbs洗5分钟
- 滴加正常山羊血清封闭液,室温20分钟,甩去多余液体
- 滴加ι抗50ul,室温静置1小时或4℃过夜或37℃1小时
- pbs洗3次各2分钟
- 滴加生物素化ⅱ抗,20℃-37℃ 20分钟
- pbs洗3次各2分钟
- 滴加试剂sabc 20℃-30℃ 20分钟
- pbs洗4次各5分钟
- dab显色:试剂盒或自配显色剂显色
- 脱水、透明、封片、镜检
冰冻切片的免疫组化染色步骤
- 速冻组织
- 冰冻切片4~8μm,冰冻切片后如不染色,必须吹干,储存低温冰箱内,或进行短暂预固定后储存于-20℃冰箱
- 室温放置30分钟后,入4℃丙酮固定10分钟,也可根据需要选择其他的固定方式
- pbs冲洗,5分钟×3次
- 用3%过氧化氢孵育5~10分钟,消除内源性过氧化物酶的活性
- pbs冲洗,5分钟×3次
- 下接石蜡切片免疫组化染色操作步骤(滴加一抗开始)
请来电咨询:021-60895578 或者添加qq:1764881598 直接索取说明书。 核糖体蛋白l23a抗体,anti-rpl23a抗体相关优质产品:
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