[30 µl]
catalog number: 26903
gene symbol: rac1
description: anti-active rac1-gtp mouse monoclonal antibody
background: small gtpases are a super-family of cellular signaling regulators. rac belongs to the rho sub-family of gtpases that regulate cell motility, cell pision, and gene transcription. gtp binding increases the activity of rac, and the hydrolysis of gtp to gdp renders it inactive. gtp hydrolysis is aided by gtpase activating proteins (gaps), while exchange of gdp for gtp is facilitated by guanine nucleotide exchange factors (gefs).
immunogen: recombinant full length protein of active rac1
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igm
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active rac1 antibody recognizes active rac1 from vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
rac activation assay. mef cells were treated with (lane 2) or without (lane 1) pdgf. cell lysates were incubated with an anti-active rac monoclonal antibody (cat # 26903) (top panel). th precipitated active rac was immunoblotted with an anti-rac rabbit polyclonal antibody (cat # 21003). the bottom panel shows the western blot with anti-rac of the cell lysates used (5% of that used in the top panel).
immunofluorescence:
immunofluorescence for the active rac using anti-active rac1-gtp mouse monoclonal antibody [26903] shows rac-gtp immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
the tissue sections were fixed with -20 ºc methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1m phosphate buffer with 0.3% triton x, and 4% bsa for 24h at room temperature. secondary antibodies were anti-mouse alexafluor488 and anti-rabbit alexafluor594 at 1:1000.
publications:
1.
cytoskeleton volume 71, issue 4, pages 257–272, april 2014
2.
neutrophil methods and protocols methods in molecular biology volume 1124, 2014, pp 251-268
3.
molecular and cellular neuroscience volume 60, may 2014, pages 10–25
4.
the journal of immunology april 14, 2014 1301155
5.
international journal of cancer volume 135, issue 11, pages 2493–2506, 1 december 2014
6.
j biol chem. 2014 may 30;289(22):15244-58
7.
mol cell biol. 2014 nov 15;34(22):4186-99
8.
plos one. 2014 sep 23;9(9):e108257
9.
cancer lett. 2015 jan 28;356(2 pt b):382-91
10.
physiological reportspublished 24 october 2014vol. 2no. e12175doi: 10.14814/phy2.12175
11.
anticancer research december 2014 vol. 34 no. 12 7111-7117
12.
cancer letters. march 1, 2015volume 358, issue 1, pages 76–84.
13.
integr biol (camb). 2014 dec 24
14.
mol neurobiol. 2015 jan 24
武汉纽斯特生物技术有限公司
18972339958
中国 武汉