mouse anti-flag tag monoclonal antibody catalog number t-03 clone name m2 specificity specifically recognizesflag peptide sequence [asp-tyr-lys-asp-asp-asp-asp-lys] the monoclonal anti-flag m2antibody detects only the target protein band(s) on a western blot from an e. coli or mammalian crude cell lysate. species/subtype mouse igg2b/k product deaaion the monoclonal anti-flagm2 antibody isa murine derived, affinity purified igg1 monoclonal antibody that binds to fusion proteins containing a flag peptide sequence. the m2 antibody will recognize a flag peptide sequence at then-terminus, met-n-terminus, c-terminus, or internal sites of a fusion protein. binding of the m2 antibody is not calcium dependent. the monoclonal anti-flag m2 antibody is useful for detection,identification, and capture of fusion proteins containing a flag peptide sequence by common immunological procedures, such as western blotting, immunofluorescence, and immunoprecipitation.application elisa, wb, ip, facs, if working concentration for most applications, dilute the anti-ha solution in an appropriate buffer to a concentration range of 0.5–5 µg/ml. determine optimaldilution buffer and dilution conditions for each specific application and method. the monoclonal anti-flag m2 antibody detects as little as 1 ng of target protein by dot blot and also shows similarsensitivity by western blot analysis packagesize 1mg/mlx0.1mlinphosphatebufferedsalinepbs),ph7.4. storage store at +4°c short term. aliquot and store at –20°c or –70 °c long term
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