[30 µl]
catalog number: 26902
gene symbol: gna13
description: anti-active gα13-gtp mouse monoclonal antibody
background: heterotrimeric g proteins are essential cellular signal transducers. gα13is one of the g proteins that could mediate cell migration and angiogenesis. other biochemical and physiological functions of gα13 are being explored.
immunogen: recombinant full length active gα13 protein
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg2b
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active gα13 monoclonal antibody recognizes active gα13 of vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
ip/wb analysis of active gα13 proteins. purified gα13 proteins were treated with gdp (lane 1) or gtpgtpγs (lane 2). after immunoprecipitation with anti-active gα13-gtp mouse monoclonal antibody (cat. # 26902), these proteins were separated by sds/page and western blotted with anti- gα13 mouse monoclonal antibody (cat. # 26004).
immunohistochemistry:
staining of mef cells (treated with pdgf) with anti- gα13-gtp monoclonal antibody showing the localization of gα13-gtp in the leading edge of migrating cells.
publications:
1.
j biol chem. 2011 sep 2;286(35):31055-61
2.
br j pharmacol. 2011 feb;162(3):733-48
3.
carcinogenesis (2010)
4.
j biol chem. 2009 oct 2;284(40):27409-15
武汉纽斯特生物技术有限公司
18972339958
中国 武汉