cluster ofdifferentiation 146 (scd146) elisa kit
target name (antigen) cluster ofdifferentiation 146 (scd146
reactivity human
methode type sandwich elisa
detection range 1.25-80 ng/ml
minimum detectionlimit 0.31 ng/ml
application elisa
catalog no. abin367358
quantity 96 tests
purpose for the quantitativedetermination of human soluble cluster of differentiation 146(scd146) concentrations
in serum, plasma tissuehomogenates.
sample type serum, plasma, tissuehomogenate
detection method colorimetric
specificity this assay has high sensitivityand excellent specificity for detection of humanscd146.
cross-reactivity(details) limited by current skills andknowledge, it is impossible for us to complete the cross-reactivitydetection
between the target antigen andall analogues for other species. therefore, cross reaction maystill exist.
sensitivity the sensitivity of this assay,or lower limit of detection (lld), was defined as the lowestprotein concentration
that could be differentiatedfrom zero. it was determined as the mean o.d value of 20 replicatesof the zero
standard added by their threestandard deviations.
material not included microplate reader capable of measuringabsorbance at 450nm, with the correction wavelength set at 540nm or570nm.
an incubator which can provide stableincubation conditions up to 37°c ± 0.5°c.
squirt bottle, manifold dispenser or automatedmicroplate washer.
absorbent paper for blotting the microtiterplate.
100ml and 500ml graduatedcylinders.
deionized or distilled water.
pipettes and pipette tips.
test tubes for dilution.
application notes the supplier is only responsible for the kititself, but not for the samples consumed during the assay. the usershould
 calculate the possible amount of thesamples used in the whole test. please reserve sufficient samplesin advance.
samples to be used within 5 days may be storedat 2-8°c, otherwise samples must be stored at -20°c (≤ 1month)
or -80°c (≤ 2 months) to avoid loss ofbioactivity and contamination.
grossly hemolyzed samples are not suitable foruse in this assay.
if the samples are not indicated in themanual, a preliminary experiment to determine the validity of thekit is necessary.
please predict the concentration beforeassaying. if values for these are not within the range of thestandard curve,
 users must determine the optimal sampledilutions for their particular experiments.
tissue or cell extraction samples prepared bychemical lysis buffer may cause unexpected elisa results due tothe
impacts of certain chemicals.
owing to the possibility of mismatchingbetween antigens from another resource and antibodies used in thissupplier's
 kits (e.g., antibody targetsconformational epitope rather than linear epitope), some native orrecombinant proteins from
other manufacturers may not be recognized bythis supplier's products.
influenced by factors including cellviability, cell number and cell sampling time, samples from cellculture supernatant
may not be recognized by the kit.
fresh samples without long time storage arerecommended for the test. otherwise, protein degradation anddenaturalization
may occur in those samples and finally lead towrong results.
comment detection wavelength: 450nminformation on standard material:some standards are from natural resource, some are recombinantprotein, but the recombinant protein will not expressed
 from baculovirus. generallyit's cho cells, but that depends.the formulation of auxiliarymaterial in the standard is
classified, but it dosen't containany poisonous substance. proclin 300 (1:3000) is used aspreservative.information on reagents:most of the stop solution are 1 n h2so4,a few is not. the formulation of wash solution is classified. noneof the
components contains (sodium)azide, thimerosal, 2-mercaptoethanol (2-me) or any other poisonousmaterials. some could
 contain bsa. for thesandwich method kits, the sample diluent, antibody diluent, enzymediluent and standard all contain
bsa.information on antibodies:the provided antibodies and their host vary in different kits. someare affinity purified, some are protein a or g purified
sample volume 50-100 µl
assay time 1 - 4.5 h
plate pre-coated,strips (12 x8)
protocol this assay employs thequantitative sandwich enzyme immunoassay technique. antibodyspecific for scd146 has been pre-coated
onto a microplate. standardsand samples are pipetted into the wells and any scd146 present isbound by the immobilized antibody.
after removing any unboundsubstances, a biotin-conjugated antibody specific for scd146 isadded to the wells. after washing,
avidin conjugated horseradishperoxidase (hrp) is added to the wells. following a wash to removeany unbound avidin-enzyme reagent,
a substrate solution is addedto the wells and color develops in proportion to the amount ofscd146 bound in the initial step. the color
development is stopped and theintensity of the color is measured.
sample collection serum: use a serum separator tube(sst) and allow samples to clot for two hours at room temperatureor overnight at 4°c before
 centrifugation for 15 minutes at 1000 ×g. remove serum and assay immediately or aliquot and store samplesat -20°c or -80°c.
 avoid repeated freeze-thawcycles.
plasma: collect plasma using edtaor heparin as an anticoagulant. centrifuge for 15 minutes at 1000 ×g at 2-8°c within 30 minutes
of collection. assay immediately or aliquotand store samples at -20°c or -80°c. avoid repeated freeze-thawcycles.
tissuehomogenates: rinse 100mg tissue with 1× pbs, homogenize in1ml of 1× pbs and store overnight at -20°c. after twofreeze-thaw
cycles to break the cell membranes, centrifugethe homogenates for 5 minutes at 5000 × g, 2-8°c. remove and assaythe supernate
immediately. alternatively, aliquot and storesamples at -20°c or -80°c. centrifuge the sample again afterthawing before the assay.
avoid repeated freeze-thaw cycles.
assay precision intra-assay precision(precision within an assay): three samples of known concentrationwere tested twenty times on one plate to
assess precision.inter-assay precision (precision between assays): three samples ofknown concentration were tested in twenty assays to assessprecision.
intra-assay: cv% less than 8%
inter-assay: cv% less than 10%
restrictions for research use only
precaution of use the stop solution provided withthis kit is an acid solution. wear eye, hand, face and clothingprotection when using this material.
handling advice the kit should not be used beyond theexpiration date on the kit label.
do not mix or substitute reagents with thosefrom other lots or sources.
if samples generate values higher than thehighest standard, dilute the samples with sample diluent and repeatthe assay.
any variation in sample diluent, operator,pipetting technique, washing technique, incubation time/temperatureand kit age can
cause variation in binding.
this assay is designed to eliminateinterference by soluble receptors, binding proteins and otherfactors present in biological
samples. until all factors have been tested inthe immunoassay, the possibility of interference cannot beexcluded.
storage 4 °c/-20 °c
storage comment may be stored at 2-8°c for upto 1 month. for long term storage, please store at -20°c. try tokeep assay plate in a sealed
aluminium foil bag and avoiddampness.
expiry date 6 months

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