specification sheet
product name: mumps grades 2 antigen
catalog #: ba-na009
agent description: mumps virus is a member of the paramyxoviridae family. there is only one serotype. the virion is pleomorphic with a roughly spherical spherical shape and a diameter of 85 to 300 um.
description: the resulting antigen preparation contains a high concentration of virus and viral components as well as some cellular material.
inactivation: mumps antigen is inactivated using gamma radiation. this procedure is effective primarily by damaging viral genetic material.
cultured in: bsc-1
strain: enders
buffer: 1969b
applications: widely used for both igg and igm detection in assays which include eia with polystyrene and latex solid phases.
storage: store at -70℃ to -100℃. repeated freezing and thawing should be avoided.
hazards: inactivated and should contain no infectious material. generally accepted good laboratory practices appropriate to biological should be employed when handling this product.
recommendations for use: this antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. this preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format.
quality control information
product name: mumps grade 2 antigen
bioar performs quality control tests to ensure each batch meets in-horse specification. it is important that each user perform titrations of antigen using their own assay as each assay format and serum release makes different performance demands on the antigen.
test:
titre: this antigen preparation is titrated using a microtitre plate based elisa. antigens are tested for reactivity with igg. the dilution of antigen which generates a singal of 1.0 o.d unit in the immunoassay is compared to that of the standard approved antigen. the result of this comparison is expressed as a percentage of the reference.
igg result: 192.1%
protein concentration: protein is determined using the dye binding assay in the microassay format. the standard curve is generated with a known concentration of igg.
rusult: 2.24 mg/ml
inativation assay: the effectiveness of inactivation is tested by inoculating a bsc-1 monlayer with antigen. the culture is manipulated using the original optional culture conditions used to manufacture the antigen.
result: no growth detected

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